390 lines
13 KiB
Python
390 lines
13 KiB
Python
from pathlib import Path
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import numpy as np
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from .layer import Layer
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# 3rd-party
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import SimpleITK as sitk
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from pyometiff import OMETIFFReader
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import mrcfile
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import transforms3d
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"""
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Convert any volumetric data to 3D numpy array with order TXYZC
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"""
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SUPPORT_EXTS = ['', '.dcm', '.DCM', '.DICOM', '.ima', '.IMA',
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'.bmp', '.BMP',
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'.PIC', '.pic',
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'.gipl', '.gipl.gz',
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'.jpg', '.JPG', '.jpeg', '.JPEG',
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'.lsm', '.LSM',
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'.tif', '.TIF', '.tiff', '.TIFF',
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'.mnc', '.MNC',
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'.mrc', '.rec',
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'.mha', '.mhd',
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'.hdf', '.h4', '.hdf4', '.he2', '.h5', '.hdf5', '.he5',
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'.nia', '.nii', '.nii.gz', '.hdr', '.img', '.img.gz',
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'.nrrd', '.nhdr',
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'.png', '.PNG',
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'.vtk',
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'.ome.tiff', '.ome.tif',
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'.mrc', '.mrc.gz', '.map', '.map.gz',
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'.gz']
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OME_EXTS = ['.ome.tiff', '.ome.tif',
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'.tif', '.TIF', '.tiff', '.TIFF']
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MRC_EXTS = ['.mrc', '.mrc.gz', '.map', '.map.gz']
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DICOM_EXTS = ['', '.dcm', '.DCM', '.DICOM', '.ima', '.IMA']
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SEQUENCE_EXTS = ['.bmp', '.BMP',
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'.jpg', '.JPG', '.jpeg', '.JPEG',
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'.tif', '.TIF', '.tiff', '.TIFF',
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'.png', '.PNG']
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def get_ext(filepath: Path) -> str:
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if filepath.name.endswith(".nii.gz"):
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return ".nii.gz"
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elif filepath.name.endswith(".img.gz"):
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return ".img.gz"
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elif filepath.name.endswith(".gipl.gz"):
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return ".gipl.gz"
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elif filepath.name.endswith(".ome.tiff"):
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return ".ome.tiff"
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elif filepath.name.endswith(".ome.tif"):
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return ".ome.tif"
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elif filepath.name.endswith(".mrc.gz"):
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return ".mrc.gz"
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elif filepath.name.endswith(".map.gz"):
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return ".map.gz"
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else:
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return filepath.suffix
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def get_file_name(filepath: Path):
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ext = get_ext(filepath)
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return filepath.name.removesuffix(ext).replace(" ", "-")
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def get_file_index(filepath: Path):
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name = get_file_name(filepath)
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digits = ""
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# Iterate through the characters in reverse order
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started = False
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for char in name[::-1]:
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if char.isdigit():
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started = True
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# If the character is a digit, add it to the digits string
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digits += char
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else:
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if started:
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# If a non-digit character is encountered, stop the loop
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break
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# Reverse the digits string to get the correct order
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last_number = digits[::-1]
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return int(last_number) if last_number != "" else 0
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def get_sequence_name(filepath: Path) -> str:
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name = get_file_name(filepath)
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index = get_file_index(filepath)
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return name.removesuffix(str(index))
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def collect_sequence(filepath: Path):
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file_dict = {}
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for f in filepath.parent.iterdir():
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if f.is_file() \
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and get_ext(filepath) == get_ext(f) \
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and get_sequence_name(filepath) == get_sequence_name(f):
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index = get_file_index(f)
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file_dict[index] = f
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for key in file_dict.copy().keys():
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if not file_dict.get(key+1) \
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and not file_dict.get(key-1):
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del file_dict[key]
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file_dict = dict(sorted(file_dict.items()))
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sequence = [str(f) for f in file_dict.values()]
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if len(sequence) == 0:
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sequence = [str(filepath)]
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return sequence
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def parse_volumetric_data(data_file: str, series_id="", progress_callback=None) -> Layer:
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"""Parse any volumetric data to numpy with shap (T,X,Y,Z,C)
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Args:
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data_file (str): file path
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series_id (str, optional): DICOM series id. Defaults to "".
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Returns:
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_type_: _description_
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"""
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data_path = Path(data_file).resolve()
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ext = get_ext(data_path)
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if progress_callback:
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progress_callback(0, "Reading the Data...")
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is_sequence = False
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if ext in SEQUENCE_EXTS:
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sequence = collect_sequence(data_path)
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if len(sequence) > 1:
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is_sequence = True
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data = None
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name = "",
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description = ""
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affine = np.identity(4)
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spacing = (1, 1, 1)
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origin = (0, 0, 0)
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direction = (1, 0, 0, 0, 1, 0, 0, 0, 1)
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# Parsing with mrcfile
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if data is None and ext in MRC_EXTS and not is_sequence:
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print("Parsing with mrcfile...")
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# TODO: much to do with mrc
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with mrcfile.open(data_path, 'r') as mrc:
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data = mrc.data
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# mrc.print_header()
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# print(data.shape)
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# print(mrc.voxel_size)
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if mrc.is_single_image():
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data = np.expand_dims(data, axis=0) # expend frame
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data = np.expand_dims(data, axis=-1) # expend Z
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data = np.expand_dims(data, axis=-1) # expend channel
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elif mrc.is_image_stack():
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data = np.expand_dims(data, axis=-1) # expend Z
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data = np.expand_dims(data, axis=-1) # expend channel
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elif mrc.is_volume():
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data = np.expand_dims(data, axis=0) # expend frame
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data = np.expand_dims(data, axis=-1) # expend channel
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elif mrc.is_volume_stack():
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data = np.expand_dims(data, axis=-1) # expend channel
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name = get_file_name(data_path)
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spacing = (mrc.voxel_size.x,
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mrc.voxel_size.y,
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mrc.voxel_size.z)
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# Parsing with OMETIFFReader
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if data is None and ext in OME_EXTS and not is_sequence:
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print("Parsing with OMETIFFReader...")
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reader = OMETIFFReader(fpath=data_path)
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ome_image, metadata, xml_metadata = reader.read()
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# TODO: some old bio-format tiff the header is not the same.
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if progress_callback:
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progress_callback(0.5, "Transpose to 'TXYZC'...")
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try:
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# print(ome_image.shape)
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# for key in metadata:
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# print(f"{key},{metadata[key]}")
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ome_order = metadata['DimOrder BF Array']
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if ome_image.ndim == 2:
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ome_order = ome_order.replace("T", "")\
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.replace("C", "").replace("Z", "")
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bioxel_order = (ome_order.index('X'),
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ome_order.index('Y'))
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data = np.transpose(ome_image, bioxel_order)
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data = np.expand_dims(data, axis=0) # expend frame
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data = np.expand_dims(data, axis=-1) # expend Z
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data = np.expand_dims(data, axis=-1) # expend channel
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elif ome_image.ndim == 3:
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# -> XYZC
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ome_order = ome_order.replace("T", "").replace("C", "")
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bioxel_order = (ome_order.index('X'),
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ome_order.index('Y'),
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ome_order.index('Z'))
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data = np.transpose(ome_image, bioxel_order)
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data = np.expand_dims(data, axis=0) # expend frame
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data = np.expand_dims(data, axis=-1) # expend channel
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elif ome_image.ndim == 4:
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# -> XYZC
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ome_order = ome_order.replace("T", "")
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bioxel_order = (ome_order.index('X'),
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ome_order.index('Y'),
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ome_order.index('Z'),
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ome_order.index('C'))
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data = np.transpose(ome_image, bioxel_order)
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data = np.expand_dims(data, axis=0) # expend frame
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elif ome_image.ndim == 5:
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# -> TXYZC
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bioxel_order = (ome_order.index('T'),
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ome_order.index('X'),
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ome_order.index('Y'),
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ome_order.index('Z'),
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ome_order.index('C'))
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data = np.transpose(ome_image, bioxel_order)
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try:
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spacing = (metadata['PhysicalSizeX'],
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metadata['PhysicalSizeY'],
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metadata['PhysicalSizeZ'])
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except:
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...
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name = get_file_name(data_path)
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except:
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...
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# Parsing with SimpleITK
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if data is None:
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print("Parsing with SimpleITK...")
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if ext in DICOM_EXTS:
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data_dirpath = data_path.parent
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reader = sitk.ImageSeriesReader()
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reader.MetaDataDictionaryArrayUpdateOn()
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reader.LoadPrivateTagsOn()
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series_files = reader.GetGDCMSeriesFileNames(
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str(data_dirpath), series_id)
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reader.SetFileNames(series_files)
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itk_image = reader.Execute()
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# for k in reader.GetMetaDataKeys(0):
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# v = reader.GetMetaData(0, k)
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# print(f'({k}) = = "{v}"')
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def get_meta(key):
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try:
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stirng = reader.GetMetaData(0, key).removesuffix(" ")
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if stirng in ["No study description",
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"No series description",
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""]:
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return None
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else:
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return stirng
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except:
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return None
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study_description = get_meta("0008|1030")
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series_description = get_meta("0008|103e")
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series_modality = get_meta("0008|0060")
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name = study_description or data_dirpath.name
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if series_description and series_modality:
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description = f"{series_description}-{series_modality}"
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elif series_description:
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description = series_description
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elif series_modality:
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description = series_modality
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else:
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description = ""
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name = name.replace(" ", "-")
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description = description.replace(" ", "-")
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elif ext in SEQUENCE_EXTS and is_sequence:
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itk_image = sitk.ReadImage(sequence)
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name = get_sequence_name(data_path)
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else:
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itk_image = sitk.ReadImage(data_path)
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name = get_file_name(data_path)
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# for key in itk_image.GetMetaDataKeys():
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# print(f"{key},{itk_image.GetMetaData(key)}")
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if progress_callback:
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progress_callback(0.5, "Transpose to 'TXYZC'...")
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if itk_image.GetDimension() == 2:
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data = sitk.GetArrayFromImage(itk_image)
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if data.ndim == 3:
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data = np.transpose(data, (1, 0, 2))
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data = np.expand_dims(data, axis=-2) # expend Z
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else:
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data = np.transpose(data)
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data = np.expand_dims(data, axis=-1) # expend Z
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data = np.expand_dims(data, axis=-1) # expend channel
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data = np.expand_dims(data, axis=0) # expend frame
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elif itk_image.GetDimension() == 3:
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if ext not in SEQUENCE_EXTS:
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itk_image = sitk.DICOMOrient(itk_image, 'RAS')
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# After sitk.DICOMOrient(), origin and direction will also orient base on LPS
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# so we need to convert them into RAS
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# affine = axis_conversion(from_forward='-Z',
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# from_up='-Y',
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# to_forward='-Z',
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# to_up='Y').to_4x4()
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affine = np.array([[-1.0000, 0.0000, 0.0000, 0.0000],
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[0.0000, -1.0000, 0.0000, 0.0000],
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[0.0000, 0.0000, 1.0000, 0.0000],
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[0.0000, 0.0000, 0.0000, 1.0000]])
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spacing = tuple(itk_image.GetSpacing())
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origin = tuple(itk_image.GetOrigin())
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direction = tuple(itk_image.GetDirection())
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data = sitk.GetArrayFromImage(itk_image)
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# transpose ijk to kji
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if data.ndim == 4:
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data = np.transpose(data, (2, 1, 0, 3))
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else:
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data = np.transpose(data)
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data = np.expand_dims(data, axis=-1) # expend channel
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data = np.expand_dims(data, axis=0) # expend frame
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elif itk_image.GetDimension() == 4:
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spacing = tuple(itk_image.GetSpacing()[:3])
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origin = tuple(itk_image.GetOrigin()[:3])
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# FIXME: not sure...
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direction = np.array(itk_image.GetDirection())
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direction = direction.reshape(3, 3) if itk_image.GetDimension() == 3 \
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else direction.reshape(4, 4)
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direction = direction[1:, 1:]
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direction = tuple(direction.flatten())
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data = sitk.GetArrayFromImage(itk_image)
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if data.ndim == 5:
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data = np.transpose(data, (0, 3, 2, 1, 4))
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else:
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data = np.transpose(data, (0, 3, 2, 1))
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data = np.expand_dims(data, axis=-1)
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if itk_image.GetDimension() > 5:
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raise Exception
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t = origin
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r = np.array(direction).reshape((3, 3))
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affine = np.dot(affine,
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transforms3d.affines.compose(t, r, [1, 1, 1]))
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meta = {
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"name": name,
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"description": description,
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"spacing": spacing,
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"affine": affine,
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"xyz_shape": data.shape[1:4],
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"frame_count": data.shape[0],
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"channel_count": data.shape[-1],
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}
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return data, meta
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