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<h1 id="future-features">Future Features</h1>
<ul>
<li>Support more volumetric data formats (.map, .txm...)</li>
<li>Generate sections</li>
<li>More segmentation methods, e.g. AI segmentation.</li>
<li>Even better shader for volumetric rendering</li>
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<h3 id="bioxels">Bioxels</h3>
<p>Bioxels is based on the RAS coordinate system, Right Aanterior Superior, which was chosen over LPS because it is more compatible with most 3D CG software coordinate systems, and is in line with the 3D artist's understanding of space.</p>
<p>All distances within Bioxels are in Units, and are specified in Meter pre unit. However, when Bioxels is imported into 3D CG software, its size in the software is not scaled by reading the Meter pre unit directly. The reason for this is that many 3D operations in software require that the primtives not be too large or too small.</p>
<h3 id="based-on-openvdb">Based on OpenVDB</h3>
<p>Bioxels is based entirely on OpenVDB for storage and rendering. The main reason for choosing OpenVDB is that as a volumetric data format, it is the fastest way to work with most CG renderers.</p>
<h3 id="based-on-geometry-nodes">Based on Geometry Nodes</h3>
<p>Bioxel Nodes relies on Blender Geometry Nodes to reconstruct and render volumetric data. Node-based operations ensure that the original data is not permanently altered during reconstruction and rendering operations. The fact that the processing is based on Geometry Nodes without any additional dependencies also ensures that Blender can open files without this plugin installed. Look for more support for OpenVDB in GeometryNodes so that Bioxel Nodes can do more in the future.</p>
<h1>Benchmark</h1>

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@@ -717,27 +1030,8 @@
<h1 id="features-options">Features &amp; Options</h1>
<h2 id="bioxel-design-concept">Bioxel Design Concept</h2>
<p>According to Bioxel design concept, Bioxel Nodes imports volume data and put it into a <strong>Container</strong> as a <strong>Layer</strong>. One container may has more than one layer, and each layer stores the information of different fields under the same location, which is similar to the view layers in map app, except that here it is in 3D space. In order to render the volume the way we want it to, we need to build renderable objects from layers. We call those objects <strong>Component</strong>. The following diagram shows the relationship of <strong>Container</strong>, <strong>Layer</strong>, and <strong>Component</strong>:</p>
<p><img alt="alt text" src="../assets/features_concept.png" /></p>
<p>Bioxel Nodes staging the layer as an openvdb file. The process of building from layers to a component is taking place in blender geometry nodes graph with blender native geometry nodes. Therefore, <strong>the blender file works without Bioxel Nodes.</strong></p>
<h2 id="container-structure">Container Structure</h2>
<p>In Blender, container structure is like this:</p>
<div class="language-bash highlight"><pre><span></span><code><span id="__span-0-1"><a id="__codelineno-0-1" name="__codelineno-0-1" href="#__codelineno-0-1"></a>Case_0000<span class="w"> </span><span class="c1"># Container</span>
</span><span id="__span-0-2"><a id="__codelineno-0-2" name="__codelineno-0-2" href="#__codelineno-0-2"></a><span class="p">|</span>--<span class="w"> </span>Case_0000_CT<span class="w"> </span><span class="c1"># Layer</span>
</span><span id="__span-0-3"><a id="__codelineno-0-3" name="__codelineno-0-3" href="#__codelineno-0-3"></a><span class="p">|</span>--<span class="w"> </span>Case_0000_Label_1<span class="w"> </span><span class="c1"># Layer</span>
</span><span id="__span-0-4"><a id="__codelineno-0-4" name="__codelineno-0-4" href="#__codelineno-0-4"></a><span class="sb">`</span>--<span class="w"> </span>Case_0000_Label_2<span class="w"> </span><span class="c1"># Layer</span>
</span></code></pre></div>
<p>The container also stores the build process in geometry nodes:</p>
<p><img alt="alt text" src="../assets/features_container.png" /></p>
<p>The layer is categorized into these by data type:</p>
<ul>
<li>Scalar</li>
<li>Label</li>
<li>Vector (Not implemented yet)</li>
<li>Color (Not implemented yet)</li>
</ul>
<h1>Import & Preprocess</h1>
<h2 id="first-time-import-volume-data">First Time Import Volume Data</h2>
<p>File &gt; Import &gt; Volume Data as Bioxel Layer</p>
<h3 id="resample">Resample</h3>
@@ -769,11 +1063,6 @@
<h2 id="adding-volume-data-to-an-existing-container">Adding Volume Data to an existing container</h2>
<p>In 3D view or outliner panel, select the container and right click, Bioxel Nodes &gt; Add Volume Data to Container.
The import settings are the same as for the first time import.</p>
<h2 id="convert-bioxel-components-to-mesh">Convert Bioxel Components to Mesh</h2>
<p>In 3D view or outliner panel, select the container and right click, Bioxel Nodes &gt; Bioxel Components To Mesh
Once it has been turned into a mesh model, you can perform any traditional 3D editing operations on it, such as sculpting, boolean, etc.</p>
<h2 id="export-biovel-layer-as-vdb">Export Biovel Layer as VDB</h2>
<p>File &gt; Export &gt; Biovel Layer as VDB</p>
@@ -803,7 +1092,7 @@ Once it has been turned into a mesh model, you can perform any traditional 3D ed
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@@ -484,18 +964,27 @@
<ul class="md-nav__list" data-md-component="toc" data-md-scrollfix>
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About
Support Multiple Formats
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<a href="#supported-format" class="md-nav__link">
<a href="#support-4d-volumetric-data" class="md-nav__link">
<span class="md-ellipsis">
Supported Format
Support 4D volumetric data
</span>
</a>
</li>
<li class="md-nav__item">
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<span class="md-ellipsis">
Support EEVEE NEXT
</span>
</a>
@@ -511,9 +1000,18 @@
</li>
<li class="md-nav__item">
<a href="#to-upgrade-add-on" class="md-nav__link">
<a href="#compatibile-to-newer-version" class="md-nav__link">
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To Upgrade Add-on
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</span>
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</li>
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<a href="#roadmap" class="md-nav__link">
<span class="md-ellipsis">
Roadmap
</span>
</a>
@@ -538,17 +1036,18 @@
<h1 id="bioxel-nodes">Bioxel Nodes</h1>
<p><img alt="Static Badge" src="https://img.shields.io/badge/Blender-orange?style=for-the-badge&amp;logo=blender&amp;logoColor=white" />
<img alt="GitHub License" src="https://img.shields.io/github/license/OmooLab/BioxelNodes?style=for-the-badge" />
<img alt="GitHub Release" src="https://img.shields.io/github/v/release/OmooLab/BioxelNodes?style=for-the-badge" />
<img alt="GitHub Repo stars" src="https://img.shields.io/github/stars/OmooLab/BioxelNodes?style=for-the-badge" /></p>
<p>Bioxel Nodes is a Blender add-on for scientific volumetric data visualization. It using Blender's powerful Geometry Nodes | Cycles to process and render volumetric data.</p>
<h2 id="about">About</h2>
<p>Before us, there have been many tutorials and add-ons for importing volumetric data into Blender. However, we found that there were many details that were not addressed in place, some scientific facts were ignored, and the volume rendering was not pretty enough. With Bioxel Nodes, you can easily import the volumetric data into Blender, and more importantly, it can quickly make a beautiful realistic rendering of it.</p>
<p>Below are some examples with Bioxel Nodes. Thanks to Cycles Render, the volumetric data can be rendered with great detail:</p>
<p>Bioxel Nodes is a Blender addon for scientific volumetric data visualization. It using Blender's powerful <strong>Geometry Nodes</strong> and <strong>Cycles</strong> to process and render volumetric data.</p>
<p><img alt="cover" src="assets/cover.png" /></p>
<p>The "Bioxel" in "Bioxel Nodes", is a combination of the words "Bio-" and "Voxel". Bioxel is a voxel that stores biological data. We are developing a toolkit around Bioxel for better biological data visualization. but before its release, we made this Blender version of bioxels toolkit first, in order to let more people to have fun with volumetric data. <a href="https://omoolab.github.io/BioxelNodes/latest/getting-started">Getting Started</a></p>
<h2 id="supported-format">Supported Format</h2>
<ul>
<li>Fantastic rendering result, also support EEVEE NEXT</li>
<li>Support multiple formats</li>
<li>Support 4D volumetric data</li>
<li>All kinds of cutters</li>
<li>Simple and powerful nodes</li>
<li>Based on blender natively, can work without addon.</li>
</ul>
<p><strong>Click <a href="https://omoolab.github.io/BioxelNodes/latest/installation">Getting Started</a> to begin your journey into visualizing volumetric data!</strong></p>
<h2 id="support-multiple-formats">Support Multiple Formats</h2>
<table>
<thead>
<tr>
@@ -594,61 +1093,38 @@
<td>✅ pass</td>
</tr>
<tr>
<td>Meta</td>
<td>.mha, .mhd</td>
<td>yet</td>
</tr>
<tr>
<td>HDF5</td>
<td>.hdf, .h4, .hdf4, .he2, .h5, .hdf5, .he5</td>
<td>✅ pass</td>
</tr>
<tr>
<td>VTK</td>
<td>.vtk</td>
<td>yet</td>
</tr>
<tr>
<td>BioRad</td>
<td>.PIC, .pic</td>
<td>yet</td>
</tr>
<tr>
<td>Gipl</td>
<td>.gipl, .gipl.gz</td>
<td>yet</td>
</tr>
<tr>
<td>LSM</td>
<td>.lsm, .LSM</td>
<td>yet</td>
</tr>
<tr>
<td>MINC</td>
<td>.mnc, .MNC</td>
<td>yet</td>
</tr>
<tr>
<td>MRC</td>
<td>.mrc, .rec</td>
<td>yet</td>
</tr>
</tbody>
</table>
<h2 id="support-4d-volumetric-data">Support 4D volumetric data</h2>
<p><img alt="4d" src="assets/4d-time.gif" /></p>
<p>🥰 4D volumetric data can also be imported into Blender.</p>
<h2 id="support-eevee-next">Support EEVEE NEXT</h2>
<p><img alt="eevee" src="assets/eevee.gif" /></p>
<p>👍 EEVEE NEXT is absolutely AWESOME! Bioxel Nodes is fully support EEVEE NEXT now! However, there are some limitations:</p>
<ol>
<li>Only one cutter supported.</li>
<li>EEVEE result is not that great as Cycles does.</li>
</ol>
<h2 id="known-limitations">Known Limitations</h2>
<ul>
<li>Sections cannot be generated (will be supported soon)</li>
<li>Time sequence volume not supported (will be supported soon)</li>
<li>Only works with Cycles CPU , Cycles GPU (OptiX), EEVEE</li>
<li>Section surface cannot be generated when convert to mesh (will be supported soon)</li>
</ul>
<h2 id="compatibile-to-newer-version">Compatibile to Newer Version</h2>
<p><strong>Updating this addon may break old files, so read the following carefully before updating</strong></p>
<p>Before updating this addon, you need to ask yourself whether this project file will be modified again or not, if it's an archived project file, I would recommend that you run <strong>Bioxel Nodes &gt; Save Staged Data</strong> to make the addon nodes permanent. In this way, there will be no potential problem with the nodes not functioning due to the addon update.</p>
<p>After the addon update, your old project files may not work either, this may be because you had executed <strong>Save Staged Data</strong>. If so, you need to execute <strong>Bioxel Nodes &gt; Relink Nodes to Addon</strong> to relink them to make sure that the addon's new functionality and the addon nodes are synchronized.</p>
<p>Also, unlike the newer versions, the older shaders are not based on OSL, so if you find that you can't render volumes, you need to turn on <strong>Open Shading Language (OSL)</strong> in the Render Settings.</p>
<h2 id="roadmap">Roadmap</h2>
<ul>
<li>Better multi-format import experience</li>
<li>One-click bake model with texture</li>
<li>AI Segmentation to Generate Labels</li>
</ul>
<h2 id="to-upgrade-add-on">To Upgrade Add-on</h2>
<p>To upgrade from an older version of the add-on to the latest, you need to do the following:</p>
<ol>
<li>Remove the old version of Bioxel Nodes at Preferences &gt; Add-ons</li>
<li>Add the new version and restart Blender.</li>
</ol>
<p>It is not support editing the same blender file across add-on versions. In order to make sure that the previous file works properly. You need to save the staged data before upgrading ( read the last section of <a href="https://omoolab.github.io/BioxelNodes/latest/getting-started/#share-your-file">Getting Started</a> ).</p>
<p>But even then, there is still no guarantee that the new version of the add-on will work on the old blender file. Therefore, it is highly recommended to open a new blender file to start the creating, not based on the old one.</p>
<p>Alternatively, objects from the old file that have nothing to do with Bioxel Nodes could be append to the new blender file.</p>
@@ -679,13 +1155,13 @@
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@@ -12,21 +12,21 @@
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@@ -82,7 +82,7 @@
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@@ -115,13 +115,13 @@
<div class="md-header__ellipsis">
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Bioxel Nodes Docs
Bioxel Nodes Handbook
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<li class="md-tabs__item md-tabs__item--active">
<a href="./" class="md-tabs__link">
Getting Started
</a>
</li>
<li class="md-tabs__item">
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Visualizating any Bio-structure
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<input class="md-nav__toggle md-toggle " type="checkbox" id="__nav_1" checked>
<input class="md-nav__toggle md-toggle " type="checkbox" id="__nav_2" checked>
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Import Volume Data
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Import & Preprocess
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Mask Methods
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Cut the Component
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Set Colors
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<a href="../misc/" class="md-nav__link">
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@@ -585,16 +934,6 @@
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Share Your File
For Blender 4.1
</span>
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@@ -663,87 +970,25 @@
<h1 id="getting-started">Getting Started</h1>
<p>Currently only support Blender 4.0 or above, make sure you have the correct version of Blender.</p>
<h2 id="add-on-installation">Add-on Installation</h2>
<h4 id="for-blender-42-or-higher">For Blender 4.2 or higher</h4>
<p>Download the <strong>Extension</strong> version <code>BioxelNodes_Extension_{version}.zip</code> from https://github.com/OmooLab/BioxelNodes/releases/latest<br />
In Blender, Edit &gt; Preferences &gt; Extensions &gt; Install from Disk, select the zip file you just downloaded.</p>
<h1 id="installation">Installation</h1>
<blockquote>
<p><strong>Currently only support Blender 4.1 or above, make sure you have the correct version of Blender.</strong></p>
</blockquote>
<h2 id="for-blender-42-or-higher">For Blender 4.2 or higher</h2>
<p>The most recommended way is to open <strong>Edit &gt; Preferences &gt; Extension</strong>, enter "bio" in the search box and click <strong>Install</strong>. since the addon is quite large (20MB) you may need to wait a while!</p>
<p><img alt="extension" src="../assets/installation_extension.png" /></p>
<p>Thats it!</p>
<blockquote>
<p>If it cannot be enable, just reboot blender.</p>
<p>If it cannot be enable, reboot blender or install again as administrator</p>
</blockquote>
<h4 id="for-blender-40-or-41">For Blender 4.0 or 4.1</h4>
<p>Also, you can do it maually. Download the <strong>Extension</strong> version <code>BioxelNodes_Extension_{version}.zip</code> from https://github.com/OmooLab/BioxelNodes/releases/latest<br />
In Blender, <strong>Edit &gt; Preferences &gt; Extensions &gt; Install from Disk</strong>, select the zip file you just downloaded.</p>
<h2 id="for-blender-41">For Blender 4.1</h2>
<p>Download the <strong>Addon</strong> version <code>BioxelNodes_Addon_{version}.zip</code> from https://github.com/OmooLab/BioxelNodes/releases/latest<br />
In Blender, Edit &gt; Preferences &gt; Add-ons &gt; Install, select the zip file you just downloaded.</p>
<p>The add-on requires a third-party python dependency called SimpleITK, click <code>Install SimpleITK</code> button below to install the dependency. After clicking, blender may get stuck, it is downloading and installing, just wait for a moment. After that, click <code>Reboot Blender</code> button.</p>
<p><img alt="dependency" src="../assets/getting-started_dependency.png" /></p>
<p><img alt="dependency" src="../assets/installation_dependency.png" /></p>
<p>This step may have failed due to network factors, just click "Set PyPI Mirror" to change the mirror.</p>
<h2 id="prepare-your-data">Prepare Your Data</h2>
<p>First you need to have your volumetric data ready. If not, you can access open research data from list below.</p>
<blockquote>
<p>Note that just because they are open and available for download does not mean you can use them for anything! Be sure to look at the description of the available scopes from website.</p>
</blockquote>
<table>
<thead>
<tr>
<th>Source</th>
<th>Object</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="https://datadryad.org">Dryad</a></td>
<td>Open Research Data</td>
</tr>
<tr>
<td><a href="https://openorganelle.janelia.org/datasets">OpenOrganelle</a></td>
<td>Cells</td>
</tr>
<tr>
<td><a href="https://www.embodi3d.com/files/category/37-medical-scans/">Embodi3D</a></td>
<td>Medical Images</td>
</tr>
<tr>
<td><a href="https://github.com/sfikas/medical-imaging-datasets">Github</a></td>
<td>Medical Images</td>
</tr>
<tr>
<td><a href="https://nhsx.github.io/open-source-imaging-data-sets/">NIHR</a></td>
<td>Medical Images</td>
</tr>
<tr>
<td><a href="http://medicaldecathlon.com/">Medical Segmentation Decathlon</a></td>
<td>Medical Images</td>
</tr>
<tr>
<td><a href="https://www.allencell.org/3d-cell-viewer.html">Allen Cell Explorer</a></td>
<td>Cells</td>
</tr>
<tr>
<td><a href="https://www.nlm.nih.gov/research/visible/visible_human.html">Visible Human Project</a></td>
<td>Medical Images</td>
</tr>
</tbody>
</table>
<h2 id="import-volume-data">Import Volume Data</h2>
<p>File &gt; Import &gt; Volume Data as Bioxel Layer, select <strong>one</strong> of the .dcm files and click on "Volume Data as Bioxel Layer" (you can also drag one of the .dcm files directly into the 3D viewport to trigger the import)</p>
<p><img alt="importing" src="../assets/getting-started_importing.png" /></p>
<p>It may take a while to read data. After finishing reading, it will pop up a dialog box. Ignore all the options, just click OK (we will tell about these options later).</p>
<p>After importing the data, the add-on will automatically add the necessary nodes to build the component. Click on the new created object in outliner, open the geometry nodes panel, set <code>Threshold</code> in "Mask by Threshold" node to 200, and then connect a Slime Shader node (Add &gt; Bioxel Nodes &gt; Slime Shader) after it. The node graph should be as following:</p>
<p><img alt="alt text" src="../assets/getting-started_graph.png" /></p>
<p>and then turned on the cycles rendering to directly see the result.</p>
<p><img alt="result" src="../assets/getting-started_result.png" /></p>
<p>You can change the "Threshold" to modify the preview model, or change the "Color", "Density" to modify the shader effect. All the parameters are straightforward, you can understand them by changing the values.</p>
<h2 id="share-your-file">Share Your File</h2>
<p>The VDB cache and the Bioxel nodes are not reachable to other computers if you don't save them. If you want to give your blender file to someone else, you will need to perform the following steps first:</p>
<ol>
<li>Save your blender file</li>
<li>Click Scene &gt; Bioxels &gt; Save All Bioxels in the properties panel.</li>
<li>Click Scene &gt; Custom Nodes &gt; Save All Custom Nodes in the properties panel.</li>
</ol>
<p><img alt="alt text" src="../assets/getting-started_share-file.png" /></p>
<p>After that, both the VDB cache and the Bioxel nodes are restored in relative locations. When you share this file with someone, pack the entire directory so that the resources will be not lost. The other person will be able to open it correctly, regardless of whether they have Bioxel Nodes installed or not.</p>
@@ -790,13 +1035,13 @@ In Blender, Edit &gt; Preferences &gt; Add-ons &gt; Install, select the zip file
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<h1>Mask Methods</h1>
<p>This type of node is responsible for culling out the extent of the component that does not need to be rendered, forming the interface between the object and the object, or the surface of the object (i.e., the interface between the object and the air)</p>
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@@ -1000,15 +1355,7 @@
<h1 id="nodes">Nodes</h1>
<h2 id="nodes-design-concept">Nodes Design Concept</h2>
<p>In order to build a component, the general process is to first use a "Mask Method" node to build the surface of the component based on its layers, and then connect to a "Assign Shader" node to add the material physical properties. Finally, if you need to cut the cross-section, then connect to a "Cut" node. The whole process is shown in the following diagram</p>
<p><img alt="alt text" src="../assets/nodes_concept.png" /></p>
<p>A typical example looks like this:</p>
<p><img alt="alt text" src="../assets/nodes_example.png" /></p>
<p>The "Mask Method" node tends to be very computationally intensive, and if it consumes too much time, then you can bake it with a "Bake" node after it (but you need to save the Blender file first).</p>
<p><img alt="alt text" src="../assets/nodes_bake.png" /></p>
<h2 id="mask-methods">Mask Methods</h2>
<p>This type of node is responsible for culling out the extent of the component that does not need to be rendered, forming the interface between the object and the object, or the surface of the object (i.e., the interface between the object and the air)</p>
<h3 id="mask-by-threshold">⬆️ Mask by Threshold</h3>
<div class="grid cards">
<ul>
@@ -1097,7 +1444,6 @@
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</div>
<h2 id="shaders">Shaders</h2>
<p>This type of node is responsible for setting material physical properties to the component to reflect the differences in rendering effects of different components.</p>
<h3 id="solid-shader">🥚 Solid Shader</h3>
<div class="grid cards">
<ul>
@@ -1218,7 +1564,6 @@
</ul>
</div>
<h2 id="cutters">Cutters</h2>
<p>This type of node is responsible for cutting components to present cross-section</p>
<h3 id="cut">🪚 Cut</h3>
<div class="grid cards">
<ul>
@@ -1237,28 +1582,6 @@
</li>
</ul>
</div>
<h3 id="plane-cutter">🧀 Plane Cutter</h3>
<div class="grid cards">
<ul>
<li>
<p><img alt="alt text" src="../assets/nodes_plane-cutter.png" /></p>
</li>
<li>
<p>Flat plane cutter, cuts off above Z-axis in local.</p>
<hr />
<p>Node Parameter:</p>
<ul>
<li><strong>Origin</strong>, <em>plane cutter position</em></li>
<li><strong>Direction</strong>, <em>plane cutter orientation</em></li>
<li>Guide<ul>
<li><strong>Show</strong>, <em>show the cutter guide</em></li>
<li><strong>Scale</strong>, <em>scale the cutter guide</em></li>
</ul>
</li>
</ul>
</li>
</ul>
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<h3 id="plane-object-cutter">🧀 Plane Object Cutter</h3>
<div class="grid cards">
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</div>
<h2 id="colors">Colors</h2>
<p>This type of node is responsible for setting the color properties of the shader</p>
<h3 id="color-presents">🎨 Color Presents</h3>
<div class="grid cards">
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